Journal: PLoS ONE
Article Title: Platelet Adhesion and Degranulation Induce Pro-Survival and Pro-Angiogenic Signalling in Ovarian Cancer Cells
doi: 10.1371/journal.pone.0026125
Figure Lengend Snippet: [ A ] Platelet adhesion to ovarian cancer cells was quantified based on the fluorescence detection of labelled platelets. Platelet adhesion to fibrinogen and BSA were used as ± controls [n = 8 + SEM, * = p<0.05 vs. BSA]. Fluorescence microscopy images of platelets adhering to A2780 [ B ] and 59M [ C ] cells under static conditions [representative of n = 3]. Ovarian cancer cells and platelets were stained for actin [green], platelets were stained specifically for CD42a [red/yellow].
Article Snippet: Phycoerythrin [PE]-labelled anti human P-selectin [mouse IgG], PE-labelled mouse IgG isotype control, and PE-labelled anti human CD42a [mouse IgG] antibodies were purchased from BD Pharmingen [San Diego, CA, USA].
Techniques: Fluorescence, Microscopy, Staining